Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE diluted at 1:1000Application
Immunoprecipitation of MGMT protein from Jurkat whole cell extracts using 5 μg of MGMT antibody . Western blot analysis was performed using MGMT antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | MGMT antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MGMT (O-6-methylguanine-DNA methyltransferase) |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MGMT. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus MonkeyRhesus Monkey (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGMT antibody 6-O-methylguanine-DNA methyltransferase antibody methylated-DNA--protein-cysteine methyltransferase antibody O6-methylguanine-DNA methyltransferase antibody O-6-methylguanine-DNA-alkyltransferase antibody methylguanine-DNA methyltransfer |
|---|---|
| Target | MGMT |
| Molecular Weight(MW) | 25 kDa (note) |
| Cellular Localization | Nucleus |
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 12% SDS PAGE diluted at 1:1000
Application
Immunoprecipitation of MGMT protein from Jurkat whole cell extracts using 5 μg of MGMT antibody . Western blot analysis was performed using MGMT antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
