WB
Western blot analysis of MHC class I expression in whole cell lysate of Raji (A), GA-10 (B), NCI-H929 (C), U-698-M (D), and HEL 92.1.7 (E)IHC
Immunoperoxidase staining was performed on human prostate tissue fixed in formalin and embedded in paraffin, revealing staining of glandular cell membranes.| Product Name | MHC Class I H-2Kb antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal antibody [AF6-88.5] to MHC Class I H-2Kb |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Clone Number | AF6-88.5 |
| Host Species | Mouse |
| Tested Applications | FACSIHC-FIP |
| Flow Cytometry: 1/10 - 1/20 Optimal dilution / concentration should be determined by the end user.: |
|
| Species Reactivity | Mouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | histocompatibility 2 K1 K region H2-K1 H2-K H-2K K-f H-2K(d) |
|---|---|
| Target | MHC Class I H-2Kb |
| Tissue Specificity | This antibody recognizes the H-2Kb. |
WB
Western blot analysis of MHC class I expression in whole cell lysate of Raji (A), GA-10 (B), NCI-H929 (C), U-698-M (D), and HEL 92.1.7 (E)
IHC
Immunoperoxidase staining was performed on human prostate tissue fixed in formalin and embedded in paraffin, revealing staining of glandular cell membranes.| Application Notes | Flow Cytometry: 1/10 - 1/20 Optimal dilution / concentration should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at +4°C or at -20°C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| Storage Buffer | Phosphate buffered saline containing 0.09% Sodium Azide (NaN3) |
