Application
MIA antibody detects MIA protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 whole cell lysate/extract of human MIA-transfected 293T cells 15 % SDS-PAGE MIA antibody dilution: 1:5000Application
Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with MIA antibody diluted at 1:500.| Product Name | MIA antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MIA (melanoma inhibitory activity)Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MIA. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (89%), Rat (91%), Rhesus Monkey (99%), Bovine (89%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | melanoma inhibitory activity CD-RAP |
|---|---|
| Target | MIA |
| Molecular Weight(MW) | 15 kDa (note) (2) |
| Cellular Localization | Secreted |
Application
MIA antibody detects MIA protein by western blot analysis. A. 30 μg 293T whole cell lysate/extract B. 30 whole cell lysate/extract of human MIA-transfected 293T cells 15 % SDS-PAGE MIA antibody dilution: 1:5000
Application
Whole cell extract (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with MIA antibody diluted at 1:500.| Positive Control | A375 , human MIA-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
