Application
Immunohistochemical analysis of paraffin-embedded human testis, using MIA3 antibody(10 μg/ml).Application
MIA3 antibody [N1], N-term detects MIA3 protein at Cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20℃ 100% MeOH for 5 min. Green: MIA3 protein stained by MIA3 antibody [N1], N-term diluted at 1:500. Blue: Hoechst 33343 staining.Application
Sample (30 ug of whole cell lysate) A: A549 B: Hep G2 5% SDS PAGE diluted at 1:1000| Product Name | MIA3 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MIA3 (melanoma inhibitory activity family, member 3)Required for collagen VII (COL7A1) secretion by loading COL7A1 into transport carriers. May participate in cargo loading of COL7A1 at endoplasmic reticulum exit sites by binding to COPII coat subunits Sec23/24 and guiding SH3-bound COL7A1 into a growing carrier. Does not play a role in global protein secretion and is apparently specific to COL7A1 cargo loading. However, it may participate in secretion of other proteins in cells that do not secrete COL7A1. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human MIA3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ARNT antibody D320 antibody FLJ21469 antibody FLJ39207 antibody KIAA0268 antibody TANGO antibody TANGO1 antibody UNQ6077 antibody MIA3 antibody melanoma inhibitory activity protein 3 antibody C219-reactive peptide antibody C219 reactive peptide |
|---|---|
| Molecular Weight(MW) | 214 kDa (note) (4) |
| Cellular Localization | Endoplasmic reticulum membrane; Single-pass type I membrane protein |
Application
Immunohistochemical analysis of paraffin-embedded human testis, using MIA3 antibody(10 μg/ml).
Application
MIA3 antibody [N1], N-term detects MIA3 protein at Cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in -20℃ 100% MeOH for 5 min. Green: MIA3 protein stained by MIA3 antibody [N1], N-term diluted at 1:500. Blue: Hoechst 33343 staining.
Application
Sample (30 ug of whole cell lysate) A: A549 B: Hep G2 5% SDS PAGE diluted at 1:1000| Positive Control | A549 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
