Application
Mib antibody detects Mib protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Mib antibody dilution: 1:50.| Product Name | Mib antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Mib (mind bomb)E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors. It thereby participates in many processes regulated by the Notch signaling pathway, such as midline cell fate specification prior to germ layer formation, patterning of sensory cell differentiation in the ear, neurogenesis of the hindbrain and commitment to a secretory fate in the intestine. Essential for early embryonic development. |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of zebrafish Mib. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-W |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Zebra Fish |
| Predicted Reactivity | Human, Mouse, Rat, Drosophila, Xenopus laevis, BovineHuman (97%), Mouse (97%), Rat (97%), Drosophila (80%), Xenopus laevis (96%), Bovine (97%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | fe47f05 wu:fe47f05 mind bomb im:7148100 cg5841 chunp6889 mib1 mib |
|---|---|
| Target | Mib |
| Molecular Weight(MW) | 113 kDa (note) |
| Cellular Localization | Cytoplasm , Cell membrane |
Application
Mib antibody detects Mib protein on zebrafish by whole mount immunohistochemical analysis. Sample: 2 days-post-fertilization zebrafish embryo. Mib antibody dilution: 1:50.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
