Application
Western Blot analysis of MID2 expression in transfected 293T cell line by MID2 polyclonal antibody. A: Non-transfected lysate. B: MID2 transfected lysate. 12% SDS PAGE diluted at 1:5000| Product Name | MID2 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MID2 (midline 2)The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to microtubular structures in the cytoplasm. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MID2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (100%), Rat (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ37715 antibody FLJ41813 antibody FXY2 antibody RNF60 antibody TRIM1 antibody MID2 antibody tripartite motif-containing protein 1 antibody RING finger protein 60 antibody tripartite motif protein 1 antibody midline defect 2 antibody midin 2 ant |
|---|---|
| Target | MID2 |
| Molecular Weight(MW) | 83 kDa (note) (2) |
| Cellular Localization | Cytoplasm , cytoskeleton |
Application
Western Blot analysis of MID2 expression in transfected 293T cell line by MID2 polyclonal antibody. A: Non-transfected lysate. B: MID2 transfected lysate. 12% SDS PAGE diluted at 1:5000| Positive Control | MID2 tansfected 293T cell |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
