| Product Name | Midkine antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MidkineMidkine exhibits neurite outgrowth-promoting activity and may play a role in nervous system development and/or maintenance. Its expression is predominant only for a short period from approximately one-half to two-thirds of the way through gestation; before and after that, it is barely detectable. Midkine was first found in differentiating mouse teratocarcinoma cells. It has neurotrophic activities and is mitogenic to certain, but not to all, fibroblast cell lines. |
| Immunogen | Recombinant full length protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| For ELISA: Use at a concentration of 0.5 μg/ml (100 μl/well) used to detect 0.2 ng/well recombinant Midkine. Biotin Conjugate use at a concentration of 0.2-0.3 μg/ml. For WB: Use at a concentration of 0.2 μg/ml used to detect 1.5 ng/lane of human recombinant Midkine under reducing and non-reducing conditions. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Target | Midkine |
|---|
| Application Notes | For ELISA: Use at a concentration of 0.5 μg/ml (100 μl/well) used to detect 0.2 ng/well recombinant Midkine. Biotin Conjugate use at a concentration of 0.2-0.3 μg/ml. For WB: Use at a concentration of 0.2 μg/ml used to detect 1.5 ng/lane of human recombinant Midkine under reducing and non-reducing conditions. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline pH 7.4 containing 0.1% Sodium Azide |
