Application
Application| Product Name | MIF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MIFMIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibit the random migration of macrophages (ref 1). Recently, MIF was rediscovered as a pituitary hormone that act as the counter-regulatory hormone for glucocorticoid action within the immune system (ref 2,3). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (ref 4). MIF plays a critical role in the host control of inflammation and immunity. |
| Immunogen | Recombinant protein - full length rat MIF protein |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPNeutWB |
| For IP: Use at a dilution of 1:400 - 1:600. For WB: Use at a dilution of 1:1,500 - 1:2,500. For Neutralizing : Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GIF antibody GLIF antibody Glycosylation inhibiting factor antibody Macrophage migration inhibitory factor antibody MIF protein antibody MMIF antibody Phenylpyruvate tautomerase antibody |
|---|---|
| Target | MIF |
Application
Application| Application Notes | For IP: Use at a dilution of 1:400 - 1:600. For WB: Use at a dilution of 1:1,500 - 1:2,500. For Neutralizing : Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate buffered saline, pH 7.2, containing 0.1% sodium azide |
