| Product Name | MIF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2Ar3] to MIFMIF (macrophage migration inhibitory factor) was one of the first cytokine activities to be discovered and was initially described as a T cell-derived factor that inhibits the random migration of macrophages (Weiser 1989). Recently, MIF was rediscovered as a pituitary hormone that act as the counterregulatory hormone for glucocorticoid action within the immune system (Bernhagen 1993, Mitchell 1995). MIF was released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secreted MIF counter-regulates the immunosuppressive effects of steroids on immune cell activation and cytokine production (Bucala 1998). MIF plays a critical role in the host control of inflammation and immunity. |
| Immunogen | E. coli-derived recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2Ar3 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| For ELISA: Use at a concentration of 4 μg/ml. For WB: Use at a concentration of 1 - 2 μg/ml. Detection limit is ~5 ng/ml under non-reducing and reducing conditions. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Protein G |
| Gene Synonyms | 4282 GLIF MMIF MIF 153620 P14174 GIF |
|---|---|
| Target | MIF |
| Tissue Specificity | Recognizes recombinant and natural human Migration Inhibitory Factor (MIF). Based on direct ELISA, shows no crossreactivity with most cytokines. |
| Application Notes | For ELISA: Use at a concentration of 4 μg/ml. For WB: Use at a concentration of 1 - 2 μg/ml. Detection limit is ~5 ng/ml under non-reducing and reducing conditions. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.2, containing 5% trehalose |
