Application
Immunofluorescence analysis of methanol-fixed HeLa, using MIF antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MIF antibody at 1:500 dilution.Application
Sample (30 µg of whole cell lysate) A: Molt-4 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ELISA detection of MIF using for capture at a concentration of 5 µg/mL and for detection at a concentration of 1.5 µg/mL.| Product Name | MIF antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MIF (macrophage migration inhibitory factor (glycosylation-inhibiting factor))This gene encodes a lymphokine involved in cell-mediated immunity, immunoregulation, and inflammation. It plays a role in the regulation of macrophage function in host defense through the suppression of anti-inflammatory effects of glucocorticoids. This lymphokine and the JAB1 protein form a complex in the cytosol near the peripheral plasma membrane, which may indicate an additional role in integrin signaling pathways. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MIF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Sheep, Rhesus Monkey, BovineRat (90%), Pig (94%), Sheep (91%), Rhesus Monkey (100%), Bovine (91%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | GIF antibody GLIF antibody MMIF antibody MIF antibody macrophage migration inhibitory factor antibody L-dopachrome isomerase antibody L-dopachrome tautomerase antibody phenylpyruvate tautomerase antibody macrophage migration inhibitory factor (glyc |
|---|---|
| Molecular Weight(MW) | 12 kDa (note) |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using MIF antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MIF antibody at 1:500 dilution.
Application
Sample (30 µg of whole cell lysate) A: Molt-4 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A:NIH-3T3 15% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ELISA detection of MIF using for capture at a concentration of 5 µg/mL and for detection at a concentration of 1.5 µg/mL.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
