Application
Western blot using GeneTex's protein-A purified Anti-MIP-1 beta (CCL4) antibody shows detection of recombinant pig MIP-1 beta (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with 4 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG at 1:20,000 for 45 min at room temperature.| Product Name | MIP1 beta Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MIP1 betaIn many species, both C-C chemokines macrophage inflammatory protein, or MIP-1 alpha (CCL3L1), and MIP-1 beta (CCL4) are structurally and functionally related CC chemokines. They are both potent chemoattractants for monocytes, which form an important component of the stroma of tumor tissue, and in humans may regulate tumor growth and associated inflammation. They participate in the host response to invading bacterial, viral, parasite and fungal pathogens by regulating the trafficking and activation state of selected subgroups of inflammatory cells e.g. macrophages, lymphocytes and NK cells. Both MIP-1 alpha and MIP-1 beta exert similar effects on monocytes, but their effect on lymphocytes differ. MIP-1 alpha selectively attracts CD8+ lymphocytes, while MIP-1 beta selectively attracts CD4+ lymphocytes. They contain the four highly conserved cysteine residues present in CC chemokines. MIP-1 beta (CCL4) has specificity for CCR5 receptors. In humans, it is also a major HIV-suppressive factor produced by CD8+ T cells. |
| Immunogen | This protein-A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with recombinant protein raised in yeast, corresponding to the 69 amino acids of the mature swine CCL4/MIP1s protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Pig |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | SCY4A CCL4 MIP1BETA MIP1B |
|---|---|
| Target | MIP-1b |
Application
Western blot using GeneTex's protein-A purified Anti-MIP-1 beta (CCL4) antibody shows detection of recombinant pig MIP-1 beta (CCL4) raised in yeast. The protein was purified and resolved by SDS-PAGE, then transferred to PVDF membrane. Membrane was blocked with 3% BSA , and probed with 4 µg/mL primary antibody overnight at 4°C. After washing, membrane was probed with IRDye800 Conjugated Goat Anti-Rabbit IgG at 1:20,000 for 45 min at room temperature.| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
| Storage Buffer | 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide |
