Application
MIS12 antibody detects MIS12 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MIS12 antibody diluted at a dilution of 1:1000.Application
Immunoprecipitation of MIS12 protein from 293T whole cell extracts using 5 μg of MIS12 antibody . Western blot analysis was performed using MIS12 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | MIS12 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MIS12 (MIS12, MIND kinetochore complex component, homolog (S. pombe))Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MIS12. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (97%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MTW1 hMis12 2510025F08Rik KNTC2AP MIS12 MIND kinetochore complex component homolog (S. pombe) |
|---|---|
| Target | MIS12 |
| Molecular Weight(MW) | 24 kDa (note) (5) |
| Cellular Localization | Nucleus , Kinetochore |
Application
MIS12 antibody detects MIS12 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with MIS12 antibody diluted at a dilution of 1:1000.
Application
Immunoprecipitation of MIS12 protein from 293T whole cell extracts using 5 μg of MIS12 antibody . Western blot analysis was performed using MIS12 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
