Application
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MITF antibody [N2C1], Internal diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:2000| Product Name | MITF antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MITF (microphthalmia-associated transcription factor)This gene encodes a transcription factor that contains both basic helix-loop-helix and leucine zipper structural features. It regulates the differentiation and development of melanocytes retinal pigment epithelium and is also responsible for pigment cell-specific transcription of the melanogenesis enzyme genes. Heterozygous mutations in the this gene cause auditory-pigmentary syndromes, such as Waardenburg syndrome type 2 and Tietz syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MITF. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Chicken, Rhesus Monkey, BovineRat (96%), Dog (99%), Pig (99%), Chicken (93%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MI antibody WS2 antibody WS2A antibody bHLHe32 antibody MITF antibody microphthalmia-associated transcription factor antibody class E basic helix-loop-helix protein 32 antibody |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (6) |
Application
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with MITF antibody [N2C1], Internal diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: HeLa 10% SDS PAGE diluted at 1:2000| Positive Control | HeLa , mouse skin |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
