Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000Application
Mitofilin antibody detects Mitofilin protein at mitochondria by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Mitofilin protein stained by Mitofilin antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Mitofilin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Mitofilin (inner membrane protein, mitochondrial (mitofilin)) |
| Immunogen | Recombinant protein encompassing a sequence within the N-terminus region of human Mitofilin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | MouseMouse (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp779P1653 antibody HMP antibody MGC111146 antibody P87 antibody P87/89 antibody P89 antibody PIG52 antibody IMMT antibody cell proliferation-inducing protein 52 antibody cell proliferation-inducing gene 4/52 protein antibody mitochondrial inn |
|---|---|
| Molecular Weight(MW) | 84 kDa (note) (4) |
| Cellular Localization | Mitochondrion inner membrane |
Application
Sample (30 ug of whole cell lysate) A: U87-MG 7.5% SDS PAGE diluted at 1:1000
Application
Mitofilin antibody detects Mitofilin protein at mitochondria by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: Mitofilin protein stained by Mitofilin antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
