Application
Western blot analysis of MKP-1/2 phosphorylation expression in heatshockK562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Product Name | MKP 1/2 (phospho Ser296/Ser318) antibody |
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| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MKP 1/2 (phospho Ser296/Ser318)The expression of DUSP1 gene is induced in human skin fibroblasts by oxidative/heat stress and growth factors. It specifies a protein with structural features similar to members of the non-receptor-type protein-tyrosine phosphatase family, and which has significant amino-acid sequence similarity to a Tyr/Ser-protein phosphatase encoded by the late gene H1 of vaccinia virus. The bacterially expressed and purified DUSP1 protein has intrinsic phosphatase activity, and specifically inactivates mitogen-activated protein (MAP) kinase in vitro by the concomitant dephosphorylation of both its phosphothreonine and phosphotyrosine residues. Furthermore, it suppresses the activation of MAP kinase by oncogenic ras in extracts of Xenopus oocytes. Thus, DUSP1 may play an important role in the human cellular response to environmental stress as well as in the negative regulation of cellular proliferation. [provided by RefSeq, Jul 2008] |
| Immunogen | A synthesized peptide derived from human MKP 1/2 around the phosphorylation site of Ser296/Ser318 |
| Modification | p-Ser296/Ser318 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| WB 1:500-1:2000, IHC 1:50-1:200: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MKP 1/2 (phospho Ser296/Ser318) Phospho dual specificity phosphatase 1 MKP 1/2 (phospho Ser296/Ser318) (phospho ) HVH1 Phospho MKP 1/2 (phospho Ser296/Ser318) MKP 1/2 (phospho Ser296/Ser318) (p) |
|---|---|
| Target | MKP 1/2 (phospho Ser296/Ser318) |
| Tissue Specificity | DUS1 |
Application
Western blot analysis of MKP-1/2 phosphorylation expression in heatshockK562 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.| Application Notes | WB 1:500-1:2000, IHC 1:50-1:200: |
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
