Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using MKP-4 antibody at 1:200 dilution.Application
Sample(30 ug whole cell lysate) A:A431 10% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using MKP-4 antibody at 1:100 dilution.| Product Name | MKP-4 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MKP-4 (dual specificity phosphatase 9)The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which is associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product shows selectivity for members of the ERK family of MAP kinases, is expressed only in placenta, kidney, and fetal liver, and is localized to the cytoplasm and nucleus. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MKP-4. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (100%), Rat (100%), Rhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MKP-4 antibody MKP4 antibody DUSP9 antibody mitogen-activated protein kinase phosphatase 4 antibody map kinase phosphatase 4 antibody serine/threonine specific protein phosphatase antibody dual specificity protein phosphatase 9 antibody dual specifi |
|---|---|
| Target | MKP-4 |
| Molecular Weight(MW) | 42 kDa (note) (2) |
| Cellular Localization | Cytoplasm |
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using MKP-4 antibody at 1:200 dilution.
Application
Sample(30 ug whole cell lysate) A:A431 10% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using MKP-4 antibody at 1:100 dilution.| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
