Application
MAP3K9 antibody detects MAP3K9 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K9 antibody diluted by 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using MLK1 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:500| Product Name | MLK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MLK1 (mitogen-activated protein kinase kinase kinase 9)Activates the JUN N-terminal pathway. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MLK1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (99%), Rat (97%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MEKK9 antibody MLK1 antibody PRKE1 antibody MAP3K9 antibody mitogen-activated protein kinase kinase kinase 9 antibody mixed lineage kinase 1 (tyr and ser/thr specificity) antibody |
|---|---|
| Target | MLK1 |
| Molecular Weight(MW) | 122 kDa (note) (3) |
Application
MAP3K9 antibody detects MAP3K9 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MAP3K9 antibody diluted by 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using MLK1 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:500| Positive Control | A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , Jurkat , Raji , NCI-H929 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
