Application
Western blot analysis of extracts from A549 cells, treated with etoposide 25uM 24h, using MMP1 (Cleaved-Phe100) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | MMP1 (cleaved Phe100) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MMP1 (cleaved Phe100)Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MMP1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CLG CLGN MMP1 MMP1 (cleaved F100) MMP1 (cleaved Phe100) MMP1 cleaved F100 MMP1 cleaved Phe100 Cleaved MMP1 (F100) Cleaved MMP1 (Phe100) |
|---|---|
| Target | MMP1 |
| Molecular Weight(MW) | 19 kDa (note) |
| Tissue Specificity | MMP1 (Cleaved-Phe100) Antibody detects endogenous levels of fragment of activated MMP1 resulting from cleavage adjacent to Phe100. |
Application
Western blot analysis of extracts from A549 cells, treated with etoposide 25uM 24h, using MMP1 (Cleaved-Phe100) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
