Application
Western blot analysis of MMP-1 in human MMP-1 over-expressing HEK293 cell lysate probed with| Product Name | MMP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MMP1Matrix metalloproteinases (MMPs), also called matrixins, belong to a family of proteases that are essential for the breakdown of extracellular matrix (ECM). They are thus important in apoptosis, tumor cell growth, invasion, and metastasis; as well as angiogenesis and wound healing. Most MMPs contain common domain structures that include a signal sequence, a propeptide, a catalytic domain, and a hemopexin-like (Hpx) domain. MMP-1, also known as collagenase-1 or interstitial collagenase, is a protein that degrades ECM. High levels of MMP1 have been found in the synovial tissues and fluids of patients with rheumatoid arthritis. Interleukin 1 Beta (IL-1 B) has been shown to stimulate the expression of MMP1, 2, 3 and 9. |
| Immunogen | Synthetic peptide corresponding to N-terminus of MMP1 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| ELISA: Use at a concentration of 0.1 - 1.0 μg/ml. IP: Use 3-5μg for 10^6 cells. WB: Use at a concentration of 0.5 - 2.0 μg/ml. Detects a band of approximately 52 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | CLG 120353 P03956 4312 CLGN MMP1 |
|---|---|
| Target | MMP1 |
| Tissue Specificity | This antibody is specific to MMP1, and does not cross react with MMP2, 3, 7, 8, 9 or 16. |
| Cellular Localization | Secreted |
Application
Western blot analysis of MMP-1 in human MMP-1 over-expressing HEK293 cell lysate probed with| Positive Control | PMA induced A431 whole cell lysate |
|---|---|
| Application Notes | ELISA: Use at a concentration of 0.1 - 1.0 μg/ml. IP: Use 3-5μg for 10^6 cells. WB: Use at a concentration of 0.5 - 2.0 μg/ml. Detects a band of approximately 52 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4ºC for short-term storage. Aliquot and store at -20°C for long-term. Avoid repeated freezing and thawing. |
| Storage Buffer | Preservative: 0.02% ProclinConstituents: PBS. pH 7.4 |
