Application
HepG2 whole cell extract and conditioned medium (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP10 antibody [N1C1] diluted at 1:1000.Application
MMP10 antibody detects MMP10 protein by western blot analysis. Non-transfected (-) and MMP10-transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP10 antibody diluted at 1:5000.Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using MMP10 antibody at 1:500 dilution.| Product Name | MMP10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MMP10 (matrix metallopeptidase 10 (stromelysin 2))Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades proteoglycans and fibronectin. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MMP10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, RatMouse (82%), Rat (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | SL-2 antibody STMY2 antibody MMP10 antibody matrix metalloproteinase-10 antibody matrix metalloproteinase 10 (stromelysin 2) antibody matrix metalloprotease 10 antibody stromelysin 2 antibody transin 2 antibody MMP-10 antibody transin-2 antibody |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) (2) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix |
Application
HepG2 whole cell extract and conditioned medium (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with MMP10 antibody [N1C1] diluted at 1:1000.
Application
MMP10 antibody detects MMP10 protein by western blot analysis. Non-transfected (-) and MMP10-transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MMP10 antibody diluted at 1:5000.
Application
Immunohistochemical analysis of paraffin-embedded human gastric cancer, using MMP10 antibody at 1:500 dilution.| Positive Control | HepG2 , human MMP10-transfected 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
