Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide 25uM 1h, using MMP12 (Cleaved-Glu106) Antibody. The lane on the right is treated with the synthesized peptide.
Rabbit polyclonal antibody to MMP12 (cleaved Glu106)May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
Immunogen
The antiserum was produced against synthesized peptide derived from human MMP12.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.:
MMP12 (Cleaved-Glu106) Antibody detects endogenous levels of fragment of activated MMP12 resulting from cleavage adjacent to Glu106.
Application
Application
Western blot analysis of extracts from NIH-3T3 cells, treated with etoposide 25uM 1h, using MMP12 (Cleaved-Glu106) Antibody. The lane on the right is treated with the synthesized peptide.
Application Notes
ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at -20 degrees Celcius. Product is stable for at least 1 year.
Storage Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.