Application
Western blot analysis of MMP-13 expression with Jurkat cell lysate.| Product Name | MMP13 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MMP-13The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-13 (also designated collagenase-3) is produced by breast carcinomas and degrades collagen type I, II, III. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCIPWB |
| Western blotting (0.5-4 µg/ml), immunoprecipitation and Immunohistochemistry (works with paraffin imbedded sections). However, the optimal concentrations should be determined individually. The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13. Reactivity to other species has not been tested.: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Bovine, Horse, RatRat (100%), Horse (98%), Bovine (98%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MMP13 Matrix metalloproteinase13 Collagenase 3 CLG3 Collagenase3 Matrix metalloproteinase 13 |
|---|---|
| Target | MMP-13 |
| Tissue Specificity | This antibody is specific for MMP-13. |
Application
Western blot analysis of MMP-13 expression with Jurkat cell lysate.| Application Notes | Western blotting (0.5-4 µg/ml), immunoprecipitation and Immunohistochemistry (works with paraffin imbedded sections). However, the optimal concentrations should be determined individually. The antibody recognizes the proenzyme (~60 kDa) and the cleaved fragments (34 kDa and 48 kDa) of MMP-13. Reactivity to other species has not been tested.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20ºC.The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal |
