Application
Western blot analysis of extracts from 293 cells, treated with etoposide 25uM 1h, using MMP14 (Cleaved-Tyr112) Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | MMP14 (cleaved Tyr112) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MMP14 (cleaved Tyr112)Seems to specifically activate progelatinase A. May thus trigger invasion by tumor cells by activating progelatinase A on the tumor cell surface. |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MMP14. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MMP14 MTMMP1 MMPX1 MT1MMP MMP14 (cleaved Y112) MMP14 (cleaved Tyr112) MMP14 cleaved Y112 MMP14 cleaved Tyr112 Cleaved MMP14 (Y112) Cleaved MMP14 (Tyr112) |
|---|---|
| Target | MMP14 |
| Molecular Weight(MW) | 53 kDa (note) |
| Tissue Specificity | MMP14 (Cleaved-Tyr112) Antibody detects endogenous levels of fragment of activated MMP14 resulting from cleavage adjacent to Tyr112. |
| Cellular Localization | Identified by mass spectrometry in melanosome fractions from stage I to stage IV. |
Application
Western blot analysis of extracts from 293 cells, treated with etoposide 25uM 1h, using MMP14 (Cleaved-Tyr112) Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:20000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
