| Product Name | MMP14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MMP14The matrix metalloproteinases (MMPs) are a family of at least eighteen secreted and membrane bound zincendopeptidases. Collectively, these enzymes can degrade all the components of the extracellular matrix, including fibrillar and non fibrillar collagens, fibronectin, laminin and basement membrane glycoproteins. In general, a signal peptide, a propeptide, and a catalytic domain containing the highly conserved zinc binding site characterizes the structure of the MMPs. In addition, fibronectin like repeats, a hinge region, and a C terminal hemopexin like domain allow categorization of MMPs into the collagenase, gelatinase, stomelysin and membrane-type MMP subfamilies. All MMPs are synthesized as proenzymes, and most of them are secreted from the cells as proenzymes. Thus, the activation of these proenzymes is a critical step that leads to extracellular matrix breakdown. MMPs are considered to play an important role in wound healing, apoptosis, bone elongation, embryo development, uterine involution, angiogenesis and tissue remodeling, and in diseases such as multiple sclerosis, Alzheimers, malignant gliomas, lupus, arthritis, periodontis, glumerulonephritis, atherosclerosis, tissue ulceration, and in cancer cell invasion and metastasis. |
| Immunogen | Synthetic peptide (Human) N-term. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 65 (pro-form) and 63 kDa (active form). MMP14 (MT1MMP) is elevated in several tumor cell lines and is constitutively produced by some normal cell lines. Treatment of cells with concanavalin A or the phorbal ester TPA stimulates production of MT1MMP in some cell types. The enzyme can be recovered in cell lysates. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MMP14 600754 P50281 MTMMP1 MMPX1 MT1MMP 4323 MT1-MMP MMP-X1 MMP X1 MT1 MMP |
|---|---|
| Target | MMP14 |
| Tissue Specificity | Rabbit Anti-MMP14, N-Terminal specifically binds to MMP14 and does not cross-react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc).Anti MMP14, N-terminal, also recognizes native MMP14 (MT1MMP). |
| Cellular Localization | Cell Membrane |
| Positive Control | Concentrated cell culture media from a stimulated human cell line |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. IHC-P: Use at an assay dependent dilution. WB: Use at a dilution of 1/2000. Detects a band of approximately 65 (pro-form) and 63 kDa (active form). MMP14 (MT1MMP) is elevated in several tumor cell lines and is constitutively produced by some normal cell lines. Treatment of cells with concanavalin A or the phorbal ester TPA stimulates production of MT1MMP in some cell types. The enzyme can be recovered in cell lysates. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 50% Glycerol, 0.01M PBS. pH 7.4 |
