Application
Western Blot: MMP2 Antibody (2C1) - Samples: Lane 1 - Human Recombinant MMP-2. Lane 2 - Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - , secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202.| Product Name | MMP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [2C1] to MMP2Loss of negative growth regulation and high invasive potential are neoplastic traits often associated with abnormal expression of matrix matalloproteinases. MMP-2, the signalling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity. |
| Immunogen | Activated recombinant human MMP-2 |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 2C1 |
| Host Species | Mouse |
| Tested Applications | ELISAICC/IFIHC-FIHC-PIPWB |
| Western Blot: 2-5 ug/ml. ELISA: 1 ug/ml. Immunocytochemistry/Immunofluorescence: 1:10-1:500. Immunohistochemistry: 5 ug/ml. Immunohistochemistry-Frozen: 5 ug/ml. Immunohistochemistry-Paraffin/Immunoprecipitation: 1:10-1:500.: |
|
| Species Reactivity | HumanMouseRatGuinea Pig |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein G |
| Gene Synonyms | TBE-1 MMP2 CLG4A P08253 CLG4 120360 MMPII 4313 MMP-II MONA TBE1 TBE 1 MMP II |
|---|---|
| Target | MMP2 |
| Tissue Specificity | This antibody is specific for pro and active MMP-2. |
| Light Chain Type | kappa |
Application
Western Blot: MMP2 Antibody (2C1) - Samples: Lane 1 - Human Recombinant MMP-2. Lane 2 - Schwann Cell Culture Medium Run on 10% SDS-PAGE, followed by electroblot to nitrocellulose and slot blot immunolabeling. Antibodies: primary - , secondary - anti-mouse-biotin. Detection: avidin-HRP. Chromagen: DAB/H202.| Positive Control | Lung, nerve, and various tumors |
|---|---|
| Application Notes | Western Blot: 2-5 ug/ml. ELISA: 1 ug/ml. Immunocytochemistry/Immunofluorescence: 1:10-1:500. Immunohistochemistry: 5 ug/ml. Immunohistochemistry-Frozen: 5 ug/ml. Immunohistochemistry-Paraffin/Immunoprecipitation: 1:10-1:500.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline containing 0.05% sodium azide |
