| Product Name | MMP24 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MMP24Matrix Metalloproteinase 24 (MMP24, MT5MMP) has been described as a proteoglycanase, which is critical in the turnover of ECM (extracellular matrix) components in the brain. MMP24 contains a candidate signal sequence, a propeptide region, a potential furin recognition motif, a zinc-binding catalytic domain, a hemopexin-like domain, a 24-residue hydrophobic region, which acts as a potential transmembrane domain, and a short cytosolic domain. Unlike other MTMMPs, MMP24 (MT5MMP) sheds from the cell surface as soluble proteinases, allowing its versatility as both a cell bound and soluble proteinase in ECM remodeling processes. MMP24 is elevated in several tumor cell lines, and is also produced by some normal cell lines. It is predominantly expressed in the brain, indicating a role in nervous system development. MMP24 is also highly expressed during embryonic development. The mouse MMP24 gene maps to chromosome 2. |
| Immunogen | Synthetic peptide corresponding to the cytoplasmic domain of mouse MMP24. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAFACSIHC-FIPWB |
| ELISA (indirect): Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. IHC (Frozen sections): Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1:2,000. This dilution is determined using a concentrated cell culture media from a stimulated cell line, an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. Higher antibody concentrations may be necessary for non-human samples. The antibody identifies a band at 65 kD (zymogen) and also the activation and breakdown products. GTX11647 also recognizes non-reduced (native) MMP24. Note: Since cell types differ greatly in the quantity of MMP24 produced, the conditioned media may require mitogen stimulation to visualize the bands by Western blotting. Treatment of cells with concanavolin-A or the phorbol ester TPA stimulates production of MMP24 in some cell types, and the enzyme can be recovered in cell lysates. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 10893 MT5MMP MMP25 MTMMP5 MT5-MMP 17391 AU040325 MT-MMP5 Mmp24 604871 Q9Y5R2 MT5 MMP MT MMP5 |
|---|---|
| Target | MMP24 |
| Tissue Specificity | Binds to MMP24 and does not cross-react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc). The cytoplasmic domain of MMP24 is detached when MTMMPs shed from the membrane, thus this antibody should not detect these forms of MMP24. |
| Cellular Localization | Cell Membrane and Secreted |
| Application Notes | ELISA (indirect): Use at an assay dependent dilution. FACS: Use at an assay dependent dilution. IHC (Frozen sections): Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1:2,000. This dilution is determined using a concentrated cell culture media from a stimulated cell line, an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. Higher antibody concentrations may be necessary for non-human samples. The antibody identifies a band at 65 kD (zymogen) and also the activation and breakdown products. GTX11647 also recognizes non-reduced (native) MMP24. Note: Since cell types differ greatly in the quantity of MMP24 produced, the conditioned media may require mitogen stimulation to visualize the bands by Western blotting. Treatment of cells with concanavolin-A or the phorbol ester TPA stimulates production of MMP24 in some cell types, and the enzyme can be recovered in cell lysates. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | 0.01M Phosphate-buffered saline, pH 7.4 containing 50% Glycerol and 0.1% Sodium azide |
