| Product Name | MMP26 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MMP26Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV collagen, fibronectin, fibrinogen and casein, and activates MMP9 by cleavage. The protein differs from most MMP family members in that it lacks a conserved C-terminal protein domain. |
| Immunogen | Synthetic peptide (Human) Hinge. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. A starting dilution of 1/5000 is recommended for chemiluminescent substrates. By immunoblotting against the reduced protein, the antibody identifies bands at 30 kDa and 18 kDa (zymogen and active MMP26). MMP26 does not appear to be produced by most normal quiescent cells, but the treatment of many cell types with the phorbol ester TPA or IL1 stimulates production of MMP26. Because of the low protein levels produced (pg/ml)concentration of cell culture media is often required to visualize the bands byimmunoblotting. Note: Higher antibody dilutions may be necessary for non-human samples. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 56547 605470 MGC126592 MGC126590 Q9NRE1 MMP26 |
|---|---|
| Target | MMP26 |
| Tissue Specificity | The antibody specifically binds to MMP26 and does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP7,etc.). |
| Cellular Localization | Secreted |
| Application Notes | WB: Use at a dilution of 1/1000. A starting dilution of 1/5000 is recommended for chemiluminescent substrates. By immunoblotting against the reduced protein, the antibody identifies bands at 30 kDa and 18 kDa (zymogen and active MMP26). MMP26 does not appear to be produced by most normal quiescent cells, but the treatment of many cell types with the phorbol ester TPA or IL1 stimulates production of MMP26. Because of the low protein levels produced (pg/ml)concentration of cell culture media is often required to visualize the bands byimmunoblotting. Note: Higher antibody dilutions may be necessary for non-human samples. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, 0.01M PBS. pH 7.4 |
