| Product Name | MMP9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [GE-213] to MMP9MMPs are a group of enzymes involved in matrix degradation. They share some important characteristics, such as a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and specific proteinase inhibitors known as tissue inhibitors of metalloproteinases (TIMPs). Reportedly, MMPs play a crucial role in tumor cell invasion and metastasis. |
| Immunogen | Full length native protein (purified) (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | GE-213 |
| Host Species | Mouse |
| Tested Applications | IHC-FIPWB |
| For IHC-Fr: Use at an assay dependent dilution. For IP: (native): Use at a concentration of 2μg/mg. For WB (Non-reducing only): Use at a concentration of 1-2μg/ml for 2hr at RT. Detects a band of 92kDa (pro form) and~86kDa (active form) kDa, which corresponds to the predicted molecular weight of MMP-9. Optimal dilutions / concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 4318 GELB CLG4B P14780 MMP9 120361 |
|---|---|
| Target | MMP9 |
| Tissue Specificity | Recognizes pro (latent) and active forms of MMP-9. Reacts with the complex (~200kDa) of MMP-9 and tissue inhibitor of metalloproteinases-1 (TIMP-1). It shows no crossreaction with pro and active forms of MMP-2. |
| Cellular Localization | Cytoplasmic |
| Positive Control | Conditioned, serum-free medium from (TPA-treated) human fibrosarcoma HT-1080 cells |
|---|---|
| Application Notes | For IHC-Fr: Use at an assay dependent dilution. For IP: (native): Use at a concentration of 2μg/mg. For WB (Non-reducing only): Use at a concentration of 1-2μg/ml for 2hr at RT. Detects a band of 92kDa (pro form) and~86kDa (active form) kDa, which corresponds to the predicted molecular weight of MMP-9. Optimal dilutions / concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS, pH7.4 containing 0.2% BSA and 0.09% sodium azide |
