Application
Immunofluorescence analysis of methanol-fixed HeLa, using MMP9 antibody at 1:200 dilution.Application
MMP9 antibody [N2C1], Internal detects MMP9 protein at cytosol on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. MMP9 antibody [N2C1], Internal dilution: 1:500.Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | MMP9 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MMP9 (matrix metallopeptidase 9 (gelatinase B, 92kDa gelatinase, 92kDa type IV collagenase))Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The enzyme encoded by this gene degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MMP9. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanBovine |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rabbit, Rhesus MonkeyMouse (82%), Rat (84%), Dog (81%), Pig (84%), Rabbit (89%), Rhesus Monkey (96%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CLG4B antibody GELB antibody MANDP2 antibody MMP-9 antibody MMP9 antibody matrix metalloproteinase 9 (gelatinase B 92kDa gelatinase 92kDa type IV collagenase) antibody macrophage gelatinase antibody matrix metalloproteinase-9 antibody 92 kDa gela |
|---|---|
| Molecular Weight(MW) | 78 kDa (note) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using MMP9 antibody at 1:200 dilution.
Application
MMP9 antibody [N2C1], Internal detects MMP9 protein at cytosol on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. MMP9 antibody [N2C1], Internal dilution: 1:500.
Application
Sample (30 µg of whole cell lysate) A: A431 B: H1299 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | A431 , H1299 , *Huh7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
