Application
Moesin antibody [C2C3], C-term detects MSN protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg BCL-1 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 7.5 % SDS-PAGE Moesin antibody [C2C3], C-term dilution: 1:1000Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Moesin antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:500.Application
Moesin antibody [C2C3], C-term detects Moesin protein at cell membrane in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Moesin antibody [C2C3], C-term diluted at 1:500.Application
Immunoprecipitation of Moesin protein from A431 whole cell extracts using 5 μg of Moesin antibody [C2C3], C-term . Western blot analysis was performed using Moesin antibody [C2C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | Moesin antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Moesin (moesin)Moesin (for membrane-organizing extension spike protein) is a member of the ERM family which includes ezrin and radixin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Moesin is localized to filopodia and other membranous protrusions that are important for cell-cell recognition and signaling and for cell movement. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human Moesin. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, Rhesus Monkey, BovinePig (98%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MSN antibody membrane-organizing extension spike protein antibody moesin antibody |
|---|---|
| Molecular Weight(MW) | 68 kDa (note) |
| Cellular Localization | Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) , Cytoplasm , cytoskeleton (By similarity) , Apical cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity) , Cell projection , microvillus membrane; Periphe |
Application
Moesin antibody [C2C3], C-term detects MSN protein by Western blot analysis. A. 30 µg GL261 whole cell lysate/extract B. 30 µg C8D30 whole cell lysate/extract C. 30 µg NIH-3T3 whole cell lysate/extract D. 30 µg BCL-1 whole cell lysate/extract E. 30 µg Raw264.7 whole cell lysate/extract F. 30 µg C2C12 whole cell lysate/extract 7.5 % SDS-PAGE Moesin antibody [C2C3], C-term dilution: 1:1000
Application
Sample (30 ug of whole cell lysate) A: NT2D1 7.5% SDS PAGE diluted at 1:1000
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed A431, using Moesin antibody (green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (red) at 1:500.
Application
Moesin antibody [C2C3], C-term detects Moesin protein at cell membrane in rat spleen by immunohistochemical analysis. Sample: Paraffin-embedded rat spleen. Moesin antibody [C2C3], C-term diluted at 1:500.
Application
Immunoprecipitation of Moesin protein from A431 whole cell extracts using 5 μg of Moesin antibody [C2C3], C-term . Western blot analysis was performed using Moesin antibody [C2C3], C-term . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | NT2D1 , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
