Application
Monoglyceride lipase antibody detects Monoglyceride lipase protein at nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Monoglyceride lipase antibody dilution: 1:500.Application
Monoglyceride lipase antibody detects Monoglyceride lipase protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Monoglyceride lipase antibody dilution: 1:500.Application
Monoglyceride lipase antibody detects MGLL protein at nucleus on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. Monoglyceride lipase antibody dilution: 1:500.Application
Sample (50 µg of whole cell lysate) A: Mouse white adipose tissue 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | Monoglyceride lipase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Monoglyceride lipase (monoglyceride lipase)Monoglyceride lipase (MGLL; EC 3.1.1.23) functions together with hormone-sensitive lipase (LIPE; MIM 151750) to hydrolyze intracellular triglyceride stores in adipocytes and other cells to fatty acids and glycerol. MGLL may also complement lipoprotein lipase (LPL; MIM 238600) in completing hydrolysis of monoglycerides resulting from degradation of lipoprotein triglycerides (Karlsson et al., 2001 [PubMed 11470505]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Monoglyceride lipase. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Pig, BovinePig (91%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | HU-K5 antibody, HUK5 antibody, MAGL antibody, MGL antibody, MGLL antibody, monoglyceride lipase antibody, monoacylglycerol lipase antibody, lysophospholipase homolog antibody |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) (3) |
Application
Monoglyceride lipase antibody detects Monoglyceride lipase protein at nucleus on mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. Monoglyceride lipase antibody dilution: 1:500.
Application
Monoglyceride lipase antibody detects Monoglyceride lipase protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. Monoglyceride lipase antibody dilution: 1:500.
Application
Monoglyceride lipase antibody detects MGLL protein at nucleus on U373 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U373 xenograft. Monoglyceride lipase antibody dilution: 1:500.
Application
Sample (50 µg of whole cell lysate) A: Mouse white adipose tissue 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample (30 µg of whole cell lysate) A: HeLa B: HepG2 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | HeLa , HepG2 , mouse adipose |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
