Application
HCT-116 whole cell and nuclear extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with mOvo2 antibody diluted at 1:1000.
Application
Mouse tissue extract (50 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with mOvo2 antibody diluted at 1:500.