Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MP1 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: 293T 15% SDS PAGE diluted at 1:1000Application
MP1 antibody detects MP1 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 15 % SDS-PAGE MP1 antibody dilution: 1:1000Application
MP1 antibody detects MP1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg Raw264.7 whole cell lysate/extract C. 30 µg C2C12 whole cell lysate/extract 15 % SDS-PAGE MP1 antibody dilution: 1:1000| Product Name | MP1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MP1 (MAPK scaffold protein 1)This gene encodes a scaffold protein that functions in the extracellular signal-regulated kinase (ERK) cascade. The protein is localized to late endosomes by the mitogen-activated protein-binding protein-interacting protein, and binds specifically to MAP kinase kinase MAP2K1/MEK1, MAP kinase MAPK3/ERK1, and MAP kinase MAPK1/ERK2. Studies of the orthologous gene in mouse indicate that it regulates late endosomal traffic and cell proliferation. Multiple transcript variants are expressed by this gene, but only one variant is thought to express a protein. [provided by RefSeq] |
| Immunogen | Full length human MP1 Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, Rhesus Monkey, BovineMouse (97%), Rat (96%), Zebrafish (86%), Xenopus laevis (85%), Chicken (98%), Rhesus Monkey (100%), Bovine (99%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MAP2K1IP1 antibody MAPBP antibody MAPKSP1 antibody MP1 antibody Ragulator3 antibody LAMTOR3 antibody mitogen-activated protein kinase kinase 1 interacting protein 1 antibody mitogen-activated protein kinase scaffold protein 1 antibody MEK binding p |
|---|---|
| Molecular Weight(MW) | 14 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MP1 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: 293T 15% SDS PAGE diluted at 1:1000
Application
MP1 antibody detects MP1 protein by Western blot analysis. A. 30 µg Rat2 whole cell lysate/extract 15 % SDS-PAGE MP1 antibody dilution: 1:1000
Application
MP1 antibody detects MP1 protein by Western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract B. 30 µg Raw264.7 whole cell lysate/extract C. 30 µg C2C12 whole cell lysate/extract 15 % SDS-PAGE MP1 antibody dilution: 1:1000| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
