Detection of MPEG1 by Western blot in LPS stimulated (15 hrs) RAW264.7 cell lysate (1), supernatant (2), rat spleen (3) and in the lysate of rMPEG1 transfected cells (4)
Rabbit polyclonal to MPEG1MPEG1, Mpg-1 (macrophage-specific gene, MSG, MPS1), is originally cloned from a macrophage cDNA library. Transcripts of Mpg-1 are found at a high level in mature human and murine macrophages and at a moderate level in certain myelomonocytic cell lines. Mpg-1-specific transcript was not detected in a wide variety of other tissues and cell lines. The predicted 669-amino acid Mpg-1-encoded protein has potential glycosylation and phosphorylation sites in addition to a signal sequence. The predicted molecular weight of the core protein is 71 to 74 kDa. Mpg-1 may share a distant ancestry to perforin. The function of Mpg-1 is unknown.
Immunogen
Recombinant protein - rat MPEG1
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
For WB: Use at a dilution of 1:2,000. Optimal dilutions/concentrations should be determined by the researcher.
Species Reactivity
MouseRat
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Detection of MPEG1 by Western blot in LPS stimulated (15 hrs) RAW264.7 cell lysate (1), supernatant (2), rat spleen (3) and in the lysate of rMPEG1 transfected cells (4)
Application Notes
For WB: Use at a dilution of 1:2,000. Optimal dilutions/concentrations should be determined by the researcher.
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles.