Application
MPI antibody detects MPI protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 10 % SDS-PAGE MPI antibody dilution: 1:1000Application
Immunohistochemical analysis of paraffin-embedded Gastric CA N87 xenograft, using MPI antibody at 1:100 dilution.Application
Sample(30μg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 10% SDS PAGE diluted at 1:1000| Product Name | MPI antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MPI (mannose phosphate isomerase)Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MPI. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Pig, Chimpanzee, BovineMouse (84%), Pig (92%), Chimpanzee (99%), Bovine (92%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | CDG1B antibody, FLJ39201 antibody, PMI antibody, PMI1 antibody, MPI antibody, mannose-6- phosphate isomerase antibody, phosphohexomutase antibody, mannose-6-phosphate isomerase antibody, phosphomannose isomerase 1 antibody, mannose phosphate isomerase anti |
|---|---|
| Target | MPI |
| Molecular Weight(MW) | 47 kDa (note) (3) |
| Cellular Localization | Cytoplasm |
Application
MPI antibody detects MPI protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract B. 30 µg Rat2 whole cell lysate/extract 10 % SDS-PAGE MPI antibody dilution: 1:1000
Application
Immunohistochemical analysis of paraffin-embedded Gastric CA N87 xenograft, using MPI antibody at 1:100 dilution.
Application
Sample(30μg whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa S3 E: Hep G2 F: MOLT4 G: Raji 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , PC-12 , Rat2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
