Application
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MPST antibody [C2C3], C-term diluted at 1:500.Application
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using MPST antibody at 1:100 dilution.Application
Sample(30 μg of whole cell lysate) A:A431 12% SDS PAGE diluted at 1:500| Product Name | MPST antibody [C2C3], C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MPST (mercaptopyruvate sulfurtransferase)This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MPST. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Xenopus laevis, Xenopus Tropicalis, Rhesus MonkeyXenopus laevis (92%), Xenopus Tropicalis (92%), Rhesus Monkey (86%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC24539 antibody, MST antibody, TST2 antibody, MPST antibody, human liver rhodanese antibody, 3-mercaptopyruvate sulfurtransferase antibody, mercaptopyruvate sulfurtransferase antibody |
|---|---|
| Molecular Weight(MW) | 33 kDa (note) (4) |
| Cellular Localization | Cytoplasm |
Application
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with MPST antibody [C2C3], C-term diluted at 1:500.
Application
Immunohistochemical analysis of paraffin-embedded CL1-5 xenograft, using MPST antibody at 1:100 dilution.
Application
Sample(30 μg of whole cell lysate) A:A431 12% SDS PAGE diluted at 1:500| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
