Application
MRCK alpha antibody detects CDC42BPA protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract 5 % SDS-PAGE MRCK alpha antibody dilution: 1:1000Application
Immunofluorescence analysis of methanol-fixed HCT116, using MRCK alpha antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MRCK alpha antibody at 1:100 dilution.| Product Name | MRCK alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MRCK alpha (CDC42 binding protein kinase alpha (DMPK-like))The protein encoded by this gene is a member of the Serine/Threonine protein kinase family. This kinase contains multiple functional domains. Its kinase domain is highly similar to that of the myotonic dystrophy protein kinase (DMPK). This kinase also contains a Rac interactive binding (CRIB) domain, and has been shown to bind CDC42. It may function as a CDC42 downstream effector mediating CDC42 induced peripheral actin formation, and promoting cytoskeletal reorganization. Multiple alternatively spliced transcript variants have been described, and the full-length nature of two of them has been reported. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MRCK alpha. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Chicken, BovineChicken (81%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp686L1738 antibody DKFZp686P1738 antibody FLJ23347 antibody KIAA0451 antibody MRCK antibody MRCKA antibody PK428 antibody CDC42BPA antibody DMPK-like alpha antibody ser-thr protein kinase PK428 antibody ser-thr protein kinase related to the m |
|---|---|
| Molecular Weight(MW) | 197 kDa (note) |
| Cellular Localization | Cytoplasm (By similarity) |
Application
MRCK alpha antibody detects CDC42BPA protein by Western blot analysis. A. 30 µg U87-MG whole cell lysate/extract 5 % SDS-PAGE MRCK alpha antibody dilution: 1:1000
Application
Immunofluorescence analysis of methanol-fixed HCT116, using MRCK alpha antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using MRCK alpha antibody at 1:100 dilution.| Positive Control | U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
