Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody diluted at 1:2000.Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Mre11 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Mre11 antibody at 1:500 dilution.Application
Mre11 antibody detects MRE11A protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract 7.5 % SDS-PAGE Mre11 antibody dilution: 1:1000Application
Mre11 antibody detects MRE11A protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5 % SDS-PAGE Mre11 antibody dilution: 1:1000Application
Immunoprecipitation of Mre11 protein from HeLa whole cell extracts using 5 μg of Mre11 antibody . Western blot analysis was performed using Mre11 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Product Name | Mre11 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Mre11 (MRE11 meiotic recombination 11 homolog A (S. cerevisiae))This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Mre11. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWBIB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | ATLD antibody, HNGS1 antibody, MRE11 antibody, MRE11B antibody, MRE11A antibody, double-strand break repair protein MRE11A antibody, MRE11 homolog A antibody, meiotic recombination 11 homolog A antibody, MRE11 homolog 1 antibody, AT-like disease antibody, |
|---|---|
| Molecular Weight(MW) | 81 kDa (note) |
| Cellular Localization | Nucleus (By similarity) |
Application
Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mre11 antibody diluted at 1:2000.
Application
Immunofluorescence analysis of paraformaldehyde-fixed A431, using Mre11 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded BT474 xenograft, using Mre11 antibody at 1:500 dilution.
Application
Mre11 antibody detects MRE11A protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract B. 30 µg NIH-3T3 whole cell lysate/extract 7.5 % SDS-PAGE Mre11 antibody dilution: 1:1000
Application
Mre11 antibody detects MRE11A protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract 7.5 % SDS-PAGE Mre11 antibody dilution: 1:1000
Application
Immunoprecipitation of Mre11 protein from HeLa whole cell extracts using 5 μg of Mre11 antibody . Western blot analysis was performed using Mre11 antibody . EasyBlot anti-Rabbit IgG was used as a secondary reagent.| Positive Control | HeLa , A431 , C8D30 , NIH-3T3 , PC-12 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
