Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membranes were blotted with MRLC2 antibody diluted at 1:5000 and competitor's antibody (SC-15370) diluted by 1:200.Application
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with MRLC2 antibody diluted at 1:1000.| Product Name | MRLC2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MRLC2 (myosin, light chain 12B, regulatory)The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MRLC2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Rhesus Monkey, BovineMouse (100%), Rat (100%), Xenopus laevis (95%), Rhesus Monkey (100%), Bovine (98%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MLC-B , MYL12B , myosin, light chain 12B, regulatory |
|---|---|
| Target | MRLC2 |
| Molecular Weight(MW) | 20 kDa (note) (3) |
Application
Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membranes were blotted with MRLC2 antibody diluted at 1:5000 and competitor's antibody (SC-15370) diluted by 1:200.
Application
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with MRLC2 antibody diluted at 1:1000.| Positive Control | K562 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative. |
