Application
Western blot analysis of extracts from 293/HeLa/COLO cells, using MRPL14 Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | MRPL14 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MRPL14Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 17p13.3. [provided by RefSeq] |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPL14. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:5000 Optimal dilutions should be determined experimentally by the end user. |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MRP-L32, MRP-L14, MRPL14, mitochondrial, 39S ribosomal protein L14, MRPL32, L14mt, MRP L32, MRP L14 |
|---|---|
| Target | MRPL14 |
| Molecular Weight(MW) | 15 kDa (note) |
| Tissue Specificity | MRPL14 Antibody detects endogenous levels of total MRPL14 protein. |
Application
Western blot analysis of extracts from 293/HeLa/COLO cells, using MRPL14 Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:5000 Optimal dilutions should be determined experimentally by the end user. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
