Application
Western blot analysis of extracts from HT-29 cells, using MRPL18 Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | MRPL18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MRPL18Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L18P ribosomal protein family. Three polymorphic sites exist in this gene, one of which is three nt in length which causes an extra aa near the N-terminus. [provided by RefSeq] |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPL18. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:1000 Optimal dilutions should be determined experimentally by the end user. |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | L18mt, mitochondrial, 39S ribosomal protein L18, MRP-L18, MRPL18, MRP L18 |
|---|---|
| Target | MRPL18 |
| Molecular Weight(MW) | 20 kDa (note) |
| Tissue Specificity | MRPL18 Antibody detects endogenous levels of total MRPL18 protein. |
Application
Western blot analysis of extracts from HT-29 cells, using MRPL18 Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:1000 Optimal dilutions should be determined experimentally by the end user. |
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| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
