Application
Western blot analysis of extracts from MCF-7/HepG2/Jurkat cells, using MRPL32 Antibody. The lane on the right is treated with the synthesized peptide.Application
Immunofluorescence analysis of A549 cells, using MRPL32 Antibody. The picture on the right is treated with the synthesized peptide.| Product Name | MRPL32 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MRPL32Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L32 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome Xp. [provided by RefSeq] |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPL32. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| IF: 1:100~1:500 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MRP-L32, L32mt, mitochondrial, MRPL32, 39S ribosomal protein L32, MRP L32 |
|---|---|
| Target | MRPL32 |
| Molecular Weight(MW) | 21 kDa (note) |
| Tissue Specificity | MRPL32 Antibody detects endogenous levels of total MRPL32 protein. |
Application
Western blot analysis of extracts from MCF-7/HepG2/Jurkat cells, using MRPL32 Antibody. The lane on the right is treated with the synthesized peptide.
Application
Immunofluorescence analysis of A549 cells, using MRPL32 Antibody. The picture on the right is treated with the synthesized peptide.| Application Notes | IF: 1:100~1:500 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
