Application
Western blot analysis of extracts from NIH-3T3/RAW264.7/A549 cells, using MRPS17 Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | MRPS17 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MRPS17Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S17P family. The encoded protein is moderately conserved between human mitochondrial and prokaryotic ribosomal proteins. Pseudogenes corresponding to this gene are found on chromosomes 1p, 3p, 6q, 14p, 18q, and Xq. [provided by RefSeq] |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPS17. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:40000 Optimal dilutions should be determined experimentally by the end user. |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | mitochondrial, 28S ribosomal protein S17, MRPS17, S17mt, MRP-S17, MRP S17 |
|---|---|
| Target | MRPS17 |
| Molecular Weight(MW) | 14 kDa (note) |
| Tissue Specificity | MRPS17 Antibody detects endogenous levels of total MRPS17 protein. |
Application
Western blot analysis of extracts from NIH-3T3/RAW264.7/A549 cells, using MRPS17 Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:40000 Optimal dilutions should be determined experimentally by the end user. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
