Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using MRPS33 Antibody. The picture on the right is treated with the synthesized peptide.
Rabbit polyclonal antibody to MRPS33Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. The 28S subunit of the mammalian mitoribosome may play a crucial and characteristic role in translation initiation. This gene encodes a 28S subunit protein that is one of the more highly conserved mitochondrial ribosomal proteins among mammals, Drosophila and C. elegans. Splice variants that differ in the 5' UTR have been found for this gene; all variants encode the same protein. Pseudogenes corresponding to this gene are found on chromosomes 1q, 4p, 4q, and 20q [provided by RefSeq]
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPS33.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCIHC-P
ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
MRPS33 MRP-S33 S33mt Mitochondrial 28S ribosomal protein S33 Mitochondrial28S ribosomal protein S33 MRP S33
Target
MRPS33
Molecular Weight(MW)
12 kDa (note)
Tissue Specificity
MRPS33 Antibody detects endogenous levels of total MRPS33 protein.
Application
Application
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using MRPS33 Antibody. The picture on the right is treated with the synthesized peptide.
Application Notes
ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at -20 degrees Celcius. Product is stable for at least 1 year.
Storage Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.