Application
Immunofluorescence analysis of methanol-fixed HeLa, using MRPS5 antibody at 1:200 dilution.Application
MRPS5 antibody detects MRPS5 protein at cytosol on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. MRPS5 antibody dilution: 1:500.Application
Sample(30 ug whole cell lysate) A:A431 B:H1299 C:HeLa S3 D:Hep G2 10% SDS PAGE diluted at 1:1000Application
MRPS5 antibody detects MRPS5 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MRPS5 antibody diluted at 1:500.| Product Name | MRPS5 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MRPS5 (mitochondrial ribosomal protein S5)Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S5P family. Pseudogenes corresponding to this gene are found on chromosomes 4q, 5q, and 18q. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MRPS5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, BovineRat (81%), Bovine (83%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MRP-S5 antibody S5mt antibody MRPS5 antibody mitochondrial 28S ribosomal protein S5 antibody 28S ribosomal protein S5 mitochondrial antibody mitochondrial ribosomal protein S5 antibody |
|---|---|
| Molecular Weight(MW) | 48 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Immunofluorescence analysis of methanol-fixed HeLa, using MRPS5 antibody at 1:200 dilution.
Application
MRPS5 antibody detects MRPS5 protein at cytosol on AGS xenograft by immunohistochemical analysis. Sample: Paraffin-embedded AGS xenograft. MRPS5 antibody dilution: 1:500.
Application
Sample(30 ug whole cell lysate) A:A431 B:H1299 C:HeLa S3 D:Hep G2 10% SDS PAGE diluted at 1:1000
Application
MRPS5 antibody detects MRPS5 protein at cytoplasm on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. MRPS5 antibody diluted at 1:500.| Positive Control | A431 , H1299 , HeLaS3 , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
