Application
WB analysis of 297T, A431, A549 and HeLa lysates using MSH2 antibody at a dilution of 1:500.Application
ICC/IF analysis of HeLa cells using MSH2 antibody at a dilution of 1:100.| Product Name | MSH2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse Monoclonal antibody [1B3-B12-G4] to MSH2This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC).When cloned,it was discovered to be a human homolog of the E. coli mismatch repair gene mutS,consistent with the characteristic alterations in microsatellite sequences (RER phenotype) found in HNPCC.Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq,Apr 2012] |
| Immunogen | Purified recombinant fragment of human MSH2 expressed in E. Coli. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 1B3-B12-G4 |
| Host Species | Mouse |
| Tested Applications | ICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNPCC mutS homolog 2 colon cancer nonpolyposis type 1 (E. coli) COCA1 FCC1 HNPCC1 LCFS2 |
|---|---|
| Target | MSH2 |
| Molecular Weight(MW) | 100kDa (note) |
| Cellular Localization | Nucleus |
Application
WB analysis of 297T, A431, A549 and HeLa lysates using MSH2 antibody at a dilution of 1:500.
Application
ICC/IF analysis of HeLa cells using MSH2 antibody at a dilution of 1:100.| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | Ascitic fluid containing 0.03% sodium azide. |
