| Product Name | MSH2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Mouse monoclonal [25D12] to MSH2MSH2 protein is involved in the initial recognition of mismatched nucleotides during the post replication mismatch repair process. Loss of MSH2 function leads to the accumulation of replication errors, which in turn may be responsible for the multiple mutations required for multistage carcinogenesis. |
| Immunogen | Recombinant full length protein (Human). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Clone Number | 25D12 |
| Host Species | Mouse |
| Tested Applications | IHC-FIHC-P |
| IHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Synonyms | HNPCC P43246 114030 FCC1 MSH2 4436 COCA1 HNPCC1 |
|---|---|
| Target | MSH2 |
| Tissue Specificity | This antibody reacts with a 102 kD protein known as MSH2 (Mismatch Repair protein 2). |
| Cellular Localization | Nuclear. |
| Light Chain Type | kappa |
| Positive Control | Tonsil |
|---|---|
| Application Notes | IHC: This antibody may be diluted to a titer of 1:10-1:25 in an ABC method. We suggest an incubation period of 60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation conditions and antibody dilutions should be determined by the user. Formalin fixed paraffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4-8 °C. |
| Storage Buffer | Tissue culture supernatant with sodium azide |
