Application
MSL3L1 antibody immunoprecipitates MSL3L1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of MSL3L1 protein by 2 μg of MSL3L1 antibody 7.5% SDS-PAGE The immunoprecipitated MSL3L1 protein was detected by MSL3L1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.Application
Sample (50 ug of whole cell lysate) A: mouse muscle 10% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:500Application
MSL3L1 antibody detects MSL3L1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MSL3L1 protein stained by MSL3L1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | MSL3L1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MSL3L1 (male-specific lethal 3 homolog (Drosophila))This gene encodes a nuclear protein and has similarity to drosophila male-specific lethal-3 gene. The drosophila protein plays a critical role in a dosage-compensation pathway, which equalizes X-linked gene expression in males and females. Thus this encoded protein is thought to play a similar function in chromatin remodeling and transcriptional regulation. This gene has been found to undergo X inactivation. There are four alternatively spliced transcript variants of this gene encoding different isoforms. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MSL3L1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Xenopus laevis, Pig, BovineRat (94%), Xenopus laevis (80%), Pig (96%), Bovine (94%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp586J1822 antibody MSL3L1 antibody MSL3 antibody MSL3-like 1 antibody male-specific lethal-3 protein-like 1 antibody male-specific lethal 3 homolog antibody male-specific lethal 3 homolog (Drosophila) antibody |
|---|---|
| Molecular Weight(MW) | 60 kDa (note) (4) |
| Cellular Localization | Nucleus |
Application
MSL3L1 antibody immunoprecipitates MSL3L1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 μg 293T whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of MSL3L1 protein by 2 μg of MSL3L1 antibody 7.5% SDS-PAGE The immunoprecipitated MSL3L1 protein was detected by MSL3L1 antibody diluted at 1:1000. EasyBlot anti-rabbit IgG was used as a secondary reagent.
Application
Sample (50 ug of whole cell lysate) A: mouse muscle 10% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE diluted at 1:500
Application
MSL3L1 antibody detects MSL3L1 protein at nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MSL3L1 protein stained by MSL3L1 antibody diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | 293T , mouse muscle |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
