Application
MTAP antibody detects MTAP protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MTAP antibody diluted at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MTAP antibody [N1C3] detects MTAP protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTAP protein stained by MTAP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.Application
Immunoprecipitation of MTAP protein from HeLa whole cell extracts using 5 μg of MTAP antibody [N1C3] . Western blot analysis was performed using MTAP antibody [N1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
Sample (50 µg of whole cell lysate) A: mouse liver 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft , using MTAP antibody at 1:500 dilution.| Product Name | MTAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MTAP (methylthioadenosine phosphorylase)This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq] |
| Immunogen | Full length human MTAP Recombinant protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | Chicken, Rhesus MonkeyChicken (81%), Rhesus Monkey (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MSAP antibody c86fus antibody MTAP antibody MTA phosphorylase antibody S-methyl-5'-thioadenosine phosphorylase antibody 5'-methylthioadenosine phosphorylase antibody MeSAdo phosphorylase antibody MTAPase antibody methylthioadenosine phosphorylase a |
|---|---|
| Molecular Weight(MW) | 31 kDa (note) |
| Cellular Localization | Cytoplasm |
Application
MTAP antibody detects MTAP protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with MTAP antibody diluted at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MTAP antibody [N1C3] detects MTAP protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: MTAP protein stained by MTAP antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.
Application
Immunoprecipitation of MTAP protein from HeLa whole cell extracts using 5 μg of MTAP antibody [N1C3] . Western blot analysis was performed using MTAP antibody [N1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
Sample (50 µg of whole cell lysate) A: mouse liver 12% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft , using MTAP antibody at 1:500 dilution.| Positive Control | A431 , HeLa , HepG2 , Mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
