Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBFA2T2 antibody at 1:200 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000| Product Name | MTGR1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to CBFA2T2 (core-binding factor, runt domain, alpha subunit 2; translocated to, 2)In acute myeloid leukemia, especially in the M2 subtype, the t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities. The translocation produces a chimeric gene made up of the 5'-region of the RUNX1 (AML1) gene fused to the 3'-region of the CBFA2T1 (MTG8) gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. The protein encoded by this gene binds to the AML1-MTG8 complex and may be important in promoting leukemogenesis. Several transcript variants are thought to exist for this gene, but the full-length natures of only three have been described. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the C-terminus region of human MTGR1. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, ChickenMouse (95%), Rat (95%), Chicken (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp313F2116 antibody EHT antibody MTGR1 antibody ZMYND3 antibody p85 antibody CBFA2T2 antibody myeloid translocation gene-related protein 1 antibody MTG8-like protein antibody MTG8-related protein 1 antibody ETO homolog on chromosome 20 antibody |
|---|---|
| Target | CBFA2T2 |
| Molecular Weight(MW) | 67 kDa (note) (3) |
| Cellular Localization | Nucleus |
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using CBFA2T2 antibody at 1:200 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20oC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
